Aggregation substance was already proved to have a role in Enterococcal biofilm formation [3, 36]. Adhesion lipoproteins are surface-associated lipoproteins involved in Enterococcal adherence, colonization and virulence [37] and are directly linked to biofilm formation as in agreement with our findings. Fibronectin/fibrinogen binding proteins also showed more than tenfold increase in biofilm stages and were suggested to have a significant role in E. faecalis associated urinary tract infections in a murine model [38] and endocarditis in rats [39]. Fibronectin binding protein mutants of community-associated MRSA losses the ability for fibronectin binding thereby preventing the establishment of biofilm in host tissue [40]. These proteins were assumed to be associated with bacterial aggregation and involve in primary attachment to a surface and hence can be considered as a suitable biofilm inhibiting target.