In general, major glycolytic enzymes and several enzymes in the citric acid cycle and pentose phosphate pathway were found to be in higher abundance in biofilm stages of SK460. Enzymes involved in rhamnose biosynthesis which forms the part of enterococcal polysaccharide antigen gene cluster was also found to be upregulated in biofilm stages. Compared to planktonic forms, Enterococcal biofilms showed a hike in stress response factors, DNA repair system, molecular chaperones, pheromone associated lipoproteins and other adhesion associated proteins. Besides, the present proteome analysis established the role of luxS mediated quorum sensing in enterococcal biofilm development.