Fig. 2 Digoxin enhanced the sensitivity of SW1990/Gem and Panc-1/Gem cells to gemcitabine. (A) The cytotoxicity of digoxin to SW1990/Gem and Panc-1/Gem cells. (B–C) Reverse effects of digoxin on drug resistance of gemcitabine in SW1990/Gem and Panc-1/Gem cells. (D–F) Colony formation assay. (G–I) Flow cytometric analysis of apoptosis. Data were expressed as mean ± SD, and the results were representative of three independent experiments. Significant differences were indicated as ***P < 0.001 vs. control group, ###P < 0.001 vs. gemcitabine group.