2.10  Quantitative real-time PCR
RNA samples were reverse transcribed to cDNA, and then quantitative PCR reaction was performed using AceQ qPCR SYBR Green Master Mix (Vazyme, Nanjing, China) in the LightCycler1 96 Real-Time PCR System (Roche, Basel, Swiss). The primer sequences used in this study were shown in Table 1.
Table 1 The sequences of primers used in the study.
Gene Forward (5′–3′) Reverse (5′–3′)
Nrf2 CAGCTTTTGGCGCAGACATT GACTGGGCTCTCGATGTGAC
Keap1 ATCGATGGCCACATCTATG GATCCTTCGTGTCAGCATTG
HO-1 CTTTCAGAAGGGCCAGGTGA GTAGACAGGGGCGAAGACTG
NQO1 GGTTTGGAGTCCCTGCCATT TTGCAGAGAGTACATGGAGCC
PRDX1 CCCACGGAGATCATTGTT CGAGATGCCTTCATCAGCCT
PRDX2 GAAGCTGTCGGACTACAAAGG TCGGTGGGGCACACAAAAG
PRDX3 GCCGTTGTCAATGGAGAGTT CAACAGCGTCAGAGTCACCT
PRDX4 AGAGGAGTGCCACTTCTACG GGAAATCTTCGCTTTGCTTAGGT
SRXN1 AGTTTTAGGGTACAGTTTGGCTAGGTATC AGTGGTACTTGTGCTAGGCAT ATTAGTAA
SOD2 TGGGGTTGGCTTGGTTTCAA GGAATAAGGCCTGTTGTTCCTTG
CAT CGGAGATTCAACACTGCCAATG TTCTTGACCGCTTTCTTCTGGA
GCLC GGACAAGAATACACCATCTCCA ATACTGCAGGCTTGGAATGTC
GCLM GGGAACCTGCTGAACTGG CTGGGTTGATTTGGGAACTC
GSR AGGAGCTGGAGAACGCTGGC CAATGGCCCAGAGCAGGCA
GSTA2 GGCTGCAGCTGGAGTAGAGT AAGGCAGGGAAGTAGCGATT
GSTA4 GGCAGCAAGGCCCAAGCTCCACT GGCCTAAAGATGTTGTAGACGG
GSTM2 ACA ACCTGTGCGGGGAATC AGCTTCAGCATTTCAGGGAGTG
GSTM3 GACTTTCCTAATCTGCCCTACCTC TTCTTCTTCAGTCTCACCACACAT
AKR1B1 TATTCACTGGCCGACTGGCTTTA GAACCACATTGCCCGACTCA
AKR1B10 GCAGGACGTGAGACTTCTACC ATCCTGCATCAATGGCCACC
AKR1C1 TAGCCTGTGAGGGAGGAAGAA TTGCCAATTTGGTGGCCTCT
AKR1C3 GGATTTGGCACCTATGCACCTC CTATATGGCGGAACCCAGCTTCTA
ALDH1A1 ACTCCCAAGCACGCTTAGTGCTC TCGTCATGTCTTAGCCAGCT
ALDH3A1 ACTGGGCGTGGTCCTCGTCATTGG GTGAGGATGGTGGGGGCTATGTAG
ABCC1 CATTGGCGAGCCTGGTAG TCGTAGGAGTGTCCGTGGAT
ABCC2 CTTGGGCTTCCTATGGCTCC ATCGAACAGCAGGGACTGTG
ABCC3 CAGAGAAGGTGCAGGTGACA CTAAAGCAGCATAGACGCCC
ABCC5 GTTCAGGAGAACTCGACCGTTGG TTTGGAAGTAGTCCGGATGGGCTT
IDH1 TGCAAAAATATCCCCCGGCT TACATCCCCATGGCAACACC
ME1 CTGCCTGTCATTCTGGATGT ACCTCTTACTCTTCTCTGCC
PGD ATTCTCAAGTTCCAAGACACCG GTGGTAAAACAGGGCATGGGA
GAPDH CTGACTTCAACAGCGACACC TGCTGTAGCCAAATTCGTTGT