Mouse experiments
All mice used in these experiments were male C57BL/6 inbred mice obtained commercially (The Jackson Laboratory). All mice were housed in pathogen-free facilities at the Washington University School of Medicine. PLX5622 was provided by Plexxikon Inc. and formulated in AIN-76A rodent diet at a dose of 1200 mg/kg by Research Diets. Mice were provided PLX5622 or control AIN-76A chow starting at 6 weeks of age for 2 weeks. For viral inoculations, mice were anesthesthetized with a cocktail of ketamine/xylazine/acepromazine, then inoculated subcutaneously via footpad injection (f.p., 50 μl) or intracranially (i.c., 10 μl) with a guided 29G needle into the brain’s third ventricle.