Figure 5 Mutations in DNAH5, DNAI1, DNAI2, and DNAH11 Affect Axonemal Localization of DNAH9
Respiratory cilia double-labeled with antibodies directed against acetylated tubulin (green) and DNAH9 (red) show colocalization of DNAH9 with acetylated tubulin at the distal part of the cilia from an unaffected control (yellow). In contrast, DNAH9 is absent or severely reduced in DNAH5, DNAI1, and DNAI2 mutant ciliary axonemes. Interestingly, in DNAH11 mutant cilia the distal localization of DNAH9 shifts from distal to a panaxonemal localization. Nuclei were stained with Hoechst33342 (blue). Scale bars represent 10 μm. For each individual, 15–20 cells were analyzed.