Slides were examined using AxioImager D1 microscope equipped with appropriate narrow-band pass filters for DAPI, Aqua, FITC, Cy3, and Cy5 fluorescence. Digital image capture and processing was carried out using the SmartCapture software (Digital Scientific UK). Ten randomly selected metaphase cells were karyotyped based on the M-FISH and DAPI-banding patterns using the SmartType Karyotyper software (Digital Scientific UK).