Figure S5 On-Target Effects Demonstrated for the TUN-8,8 Analogue, Related to Figure 5
(A) Glucosamine incorporation within 48 h was reduced with 10x TUN-8,8. Positive controls were tunicamycin, meropenem/clavulanate (MCA) and D-cycloserine (DCS). All compounds tested at 1- and 10-fold MIC concentrations (n=2).
(B) GFP release assay. Mtb expressing GFP was treated with 1- and 10-fold MIC concentrations of TUN-8,8 or tunicamycin. Lysis was monitored by measurement of fluorescence in cell-free supernatants daily over a week of exposure as a measure of release of cytosolic protein (GFP) (n=2).
(C) Confocal microscopy of BODIPY-vancomycin used to track synthesis of PG, showing abnormal PG formation with tunicamycin and TUN-8,8 treated Mtb, both differing from the effects of pentapeptide labelling by the BODIPY-vancomycin caused by meropenem/clavulanate treatment.
Data presented in (A) and (B) are means ± SD.