Figure S4 Kirby-Bauer Disc Diffusion Tests and Dose Response Curves Used for MIC, MBC, and IC50 Determination for Tunicamycin and the TUN-X,X Analogues against Several Bacterial Strains, Related to Figure 5
(A–E) Kirby-Bauer disc diffusion tests for the TUN analogues against bacterial strains (A) B. subtilis EC 1524, (B) M. luteus, (C) B. cereus ATCC 11778, (D) S. aureus ATCC 29219 and (E) P. aeruginosa ATCC 27853. Discs impregnated with 5 μg, unless otherwise indicated, of the compound were laid onto plates with lawns of bacteria. The compounds are labelled: TC: commercial tunicamycin, TS: tunicamycin from S. chartreusis, 2: (2) N-acetyl tunicamine, Ac: TUN-Ac,Ac, Boc: TUN-Boc,Boc, 7: TUN-7,7, 8: TUN-8,8, 9: TUN-9,9, 10: TUN-10,10, 11: TUN-11,11, 12: TUN-12,12, Cit: TUN-Cit,Cit, Amp: ampicillin, Gen: gentamycin.
(F–J) Dose response curves for tunicamycin and the analogues with (F) B. subtilis EC1524. (G) B. cereus ATCC11778. (H) S. aureus ATCC29219. (I) P. aeruginosa ATCC27853 and (J) E. coli ATCC25922. These dose-response curves were generated by Prism 6.0 software by plotting percent growth (normalized OD600 values) vs. logarithmic scale of the concentrations. The data shown are mean ± SEM errors of three independent experiments. See Table S3 for the MIC, MBC and IC50 values.