Figure 2 DPAGT1 Active Site
(A) The loops that form the active site; UDP-GlcNAc in magenta.
(B) Sliced molecular surface showing occluded active site cleft and putative Dol-P recognition groove. Surface is coloured by electrostatic potential; UDP-GlcNAc in magenta.
(C) Conformational changes with UDP-GlcNAc binding. Protein depicted in tube form with the tube thickness and colouring reflecting the rmsd in mainchain atomic positions between the unbound and UDP-GlcNAc-bound structures.
(D) UDP-GlcNAc binding in active site. Omit Fo-Fc difference electron density shown for UDP-GlcNAc (green mesh, contoured at 3σ) and 4 Å anomalous difference Fourier electron density (magenta mesh, contoured at 15σ) from a dataset with MnCl2.
(E) Recognition of uridine moiety of UDP-GlcNAc.
(F) Schematic representation of interactions made by UDP-GlcNAc.
(G) Tunicamycin binding in active site.
(H) Schematic representation of interactions made by tunicamycin.