Source Methoda Species Expression/pmol mg−1 Luminal Totalb Abluminal Relative to microvessel protein Dick et al. [303] Cytochalasin B binding Rat, pig 69–80 Kalaria et al. [571] Cytochalasin B binding Human 42 Farrell and Pardridge [572] Immunogold e.m Rat 12%c 100%c 48%c Cornford et al. [573] Immunogold e.m Human 48% 100% 18% Vannucci et al. [574] Cytochalasin B binding Rat 40–125 Kamiie et al. [182] Proteomics Mouse 90 Uchida et al. [184] Proteomics Human 139 Shawahna et al. [176] Proteomics Human 78.5 Hoshi et al. [185] Lysate digestion proteomics Rat 84–98 Relative to membrane protein Simpson et al. [575] Fractionation, cytochalasin B binding Bovine 620 280 Kubo et al. [58] Fractionation, proteomics Porcine 79% 21% Zhang et al. [188] Proteomics Porcine 300