Following computational and manual curation, variants in 101 genes were reclassified in the DVD. These reclassifications included major categorical changes representing medically significant changes (P/LP versus VUS/LB/B) for 300 variants in 52 genes (Table S3). Of the 20% of genes carrying the greatest number of medically significant differences, six are associated with the diagnosis of Usher syndrome (Figure 2G, Table S4). For both ClinVar and HGMD, the same five genes carry the greatest number of major categorical changes (USH2A [MIM: 608400], SLC26A4 [MIM: 605646], GJB2, MYO7A [MIM: 276903], CDH23 [MIM: 605516]) (Figure 2G, Table S4). The remaining frequently impacted genes are WFS1 (MIM: 606201) (DFNA6/14/38 [MIM: 600965] and Wolfram syndrome), USH1C (MIM: 605242) (DFNB18A [MIM: 602092] and USH1C [MIM: 276904]), ADGRV1 (MIM: 602851) (USH2C [MIM: 605472]), and MYO15A (MIM: 602666) (DFNB3 [MIM: 600316]).