(C) Alignment of the three in-frame NDUFA6 transcripts demonstrates that ENST00000602404 (GenBank: NM_002490.5) uses an initiator methionine downstream of that used by ENST00000498737 (GenBank: NM_002490.4). The shortest transcript, ENST00000470753, initiates downstream of the highly conserved LYR motif (shaded yellow) that is reported to be critical for binding the mitochondrial acyl carrier (encoded by NDUFAB1); the function of this isoform therefore remains unknown. Subject variants are shaded blue.