Figure 1 Modifiers of Serine-ADP-Ribosylation of Histone Peptides
(A) Autoradiogram showing ADPr, and subsequent ARH3-mediated glycohydrolysis of H3 1–20aa, H3 27–45aa, H2A 1–17aa, and H4 1–23aa peptides. Coomassie staining of the SDS-PAGE is included and represents the loading control.
(B) Autoradiogram showing PARP1/2 + HPF1-mediated ADPr of H3 peptide with Lys9 substituted by Ala and Arg, and Ser10 substituted by Ala. Coomassie staining of the SDS-PAGE is included.
(C) 293T cells were transfected with the same amount of empty vector (EV) or plasmid expressing WT, K9A, K9R, or S10A FLAG-tagged histone H3 protein and treated for 10 min with H2O2. Inputs (A) and FLAG-IPs (B) were analyzed by western blotting.