For 411 samples, the Illumina TruSight Cancer Panel (TCP) was also used (gene list in Table S2), and libraries were sequenced with an Illumina MiSeq. BCL files resulting from the sequencing were converted to FASTQ files with Illumina’s bcl2fastq. FASTQ files were checked for coverage and other quality-control parameters with fastqc software. FASTQ files were aligned to the UCSC Genome Browser (hg19) with the Burrows-Wheeler Aligner (BWA-MEM) with default parameters and SAMtools for the generation of a binary compressed sequence alignment map (BAM) files.11, 12 Variants were called from BAM files with the Genome Analysis Toolkit Unified Genotyper algorithm.13, 14 All data were annotated with Variant Effect Predictor (VEP) v.87 on the basis of canonical transcripts.15