RNA Isolation and RT-PCR in Zebrafish Embryos
Trizol (ThermoFisher Scientific) was used to isolate RNA from zebrafish embryos according to the manufacturer’s instructions. Approximately 30 embryos for each experimental group were pooled for RNA isolation. The SuperScript III First-Strand Synthesis System (ThermoFisher Scientific) was used to produce cDNA from the isolated RNA according to the manufacturer’s instructions. RT-PCR was performed with the primers listed in Table S1.