Figure 3  Effects of LBP on neuronal viability and mortality. (A) Immunochemistry staining showing that the majority of the cultured cells are neurons (MAP2 positive cells, red) with few astrocytes (GFAP positive cells, green). (B) Two slopes showing the relationship between neuronal viability (red slope), death (blue slope) and oxygen-glucose deprivation (OGD) duration. (C) Immunochemistry staining showing effects of LBP treatment on morphological changes of neurons exposed to 4 h OGD. Neurons exhibited features of beaded dendrites in the OGD group (C3, arrows). However, LBP recovered normal dendritic morphology (C4, arrow). (D) Fluoro JADE B (FJB) staining (injury marker) showing strong fluorescent labeling in most neurons when exposed to OGD for 4 h (D3, arrows). However, LBP treatment reduced the staining intensity (D4, arrow). (E) Quantitative results showing effects of LBP treatment on neuronal injury (FJB staining), mortality and viability when exposed to 4 h OGD (**P < 0.01). The scale bar in (D4) is equal to (C1–C4) and (D1–D3).