PMC:5590178 / 9236-10133 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/5590178","sourcedb":"PMC","sourceid":"5590178","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5590178","text":"Table 1 Sequences of primers (5′ → 3′) used to construct plasmids encoding various human GFAP alleles\nAllele Sequences\nWT Forward: TAGTAGGATCCATGGAGAGGAGACGCATCAC\nReverse: TAGTCGATATCATCATCACATCCTTGTGCTCCTGCTTG\np.Arg79Cys Forward: GAGATGATGGAGCTCAATGACtGCTTTGCCAGCTACATCGAG\nReverse: CTCGATGTAGCTGGCAAAGCaGTCATTGAGCTCCATCATCTC\np.Arg79His Forward: GAGATGATGGAGCTCAATGACCaCTTTGCCAGCTACATCGAG\nReverse: CTCGATGTAGCTGGCAAAGtGGTCATTGAGCTCCATCATCTC\np.Asp128Asn Forward: GAGAGCTGCGGCTGCGGCTCaATCAACTCACCGCCAACAG\nReverse: CTGTTGGCGGTGAGTTGATtGAGCCGCAGCCGCAGCTCTC\np.Asp157Asn Forward: GCAGAAGCTCCAGaATGAAACCAACCTG\nReverse: CAGGTTGGTTTCATtCTGGAGCTTCTGC\np.Arg239Cys Forward: CAGCCCTGAAAGAGATCtGCACGCAGTATGAGGCAATG\nReverse: CATTGCCTCATACTGCGTGCaGATCTCTTTCAGGGCTG\np.Arg239His Forward: CAGCCCTGAAAGAGATCCaCACGCAGTATGAGGCAATG\nReverse: CATTGCCTCATACTGCGTGtGGATCTCTTTCAGGGCTG\nLower case indicates mutated nucleotides","divisions":[{"label":"label","span":{"begin":0,"end":7}},{"label":"caption","span":{"begin":8,"end":101}},{"label":"p","span":{"begin":8,"end":101}},{"label":"table","span":{"begin":102,"end":856}},{"label":"tr","span":{"begin":102,"end":118}},{"label":"th","span":{"begin":102,"end":108}},{"label":"th","span":{"begin":109,"end":118}},{"label":"tr","span":{"begin":119,"end":162}},{"label":"td","span":{"begin":119,"end":121}},{"label":"td","span":{"begin":122,"end":162}},{"label":"tr","span":{"begin":163,"end":210}},{"label":"td","span":{"begin":163,"end":210}},{"label":"tr","span":{"begin":211,"end":273}},{"label":"td","span":{"begin":211,"end":221}},{"label":"td","span":{"begin":222,"end":273}},{"label":"tr","span":{"begin":274,"end":325}},{"label":"td","span":{"begin":274,"end":325}},{"label":"tr","span":{"begin":326,"end":388}},{"label":"td","span":{"begin":326,"end":336}},{"label":"td","span":{"begin":337,"end":388}},{"label":"tr","span":{"begin":389,"end":440}},{"label":"td","span":{"begin":389,"end":440}},{"label":"tr","span":{"begin":441,"end":502}},{"label":"td","span":{"begin":441,"end":452}},{"label":"td","span":{"begin":453,"end":502}},{"label":"tr","span":{"begin":503,"end":552}},{"label":"td","span":{"begin":503,"end":552}},{"label":"tr","span":{"begin":553,"end":602}},{"label":"td","span":{"begin":553,"end":564}},{"label":"td","span":{"begin":565,"end":602}},{"label":"tr","span":{"begin":603,"end":640}},{"label":"td","span":{"begin":603,"end":640}},{"label":"tr","span":{"begin":641,"end":700}},{"label":"td","span":{"begin":641,"end":652}},{"label":"td","span":{"begin":653,"end":700}},{"label":"tr","span":{"begin":701,"end":748}},{"label":"td","span":{"begin":701,"end":748}},{"label":"tr","span":{"begin":749,"end":808}},{"label":"td","span":{"begin":749,"end":760}},{"label":"td","span":{"begin":761,"end":808}},{"label":"tr","span":{"begin":809,"end":856}},{"label":"td","span":{"begin":809,"end":856}}],"tracks":[]}