As an additional demonstration of the duplication phenomenon, we considered the occurrence of Pfam motifs within ORFs, with only the best Pfam match retained per ORF (around 1,930 of the 2,011 Pfam categories were represented). Matching successive runs of four or more (that occur at least three times on the genome) appear in the additional data files. Many of the runs occur on the near-diagonal. Most involve four identical Pfam categories in succession, or a double run of two categories, again pointing to local duplication.