Using a stereomicroscope, the live image of each brain section was evaluated according to the rat brain atlas. The volume "V(mPFC)" was estimated using the Cavalieri method (Kristiansen and Nyengaard, 2012[14]). The sum of the area of the favored structure (ΣA) (mPFC, ACC, PLC, and ILC) was estimated using the software designed at the University. The distance between the sampled sections was defined as “d”. Finally, the volume of each structure was estimated by the following formula: