Figure 2  Scrib interacts with LPP in mammalian cells. pM-bait- and pSNATCH-prey-constructs were cotransfected into 293 cells in the combination indicated, together with a GAL4-regulated luciferase reporter and a CMV-β-galactosidase internal control. Cell lysates were assayed for luciferase activity 18–24 hours after transfection. Relative luciferase activity is reported as the average of three independent duplo experiments (with standard error).