Protein expression analysis
All protein expressions measured in perfusate were adjusted to the predicted total lung capacity (pTLC) of the donor as an estimate of perfused donor lung volume and were reported as corrected perfusate concentrations (pg/ml). The pTLC was calculated in a routine fashion based on donor gender and height [18]. If one lung was deemed unusable due to severe consolidation or extensive contusion on inspection, or if the intended recipient required a single lung transplant on a specific side, only one lung was procured. For single-lung perfusions, the pTLC was adjusted to a factor of 0.55 for right lung and 0.45 for left lung perfusion [19].