Architectural differences in the epidermis made Western blot analyses somewhat difficult to gauge. However, in regions such as ear skin, where the highest levels of Snail protein were expressed, the effects were accentuated. In both back skin and ear skin, overall levels of E-cadherin and α-catenin were reduced, under conditions where β-catenin and Ajuba levels remained unchanged relative to controls (Figure 4B). Taken together, these data were consistent with our results obtained from immunofluorescence microscopy.