TrkA/TrkC and PV Immunohistochemistry in DRG and Spinal Cord
Red represents TrkA, green represents TrkC (and PV in [C]), and yellow represents co-expression.
(A) TrkA/TrkC immunostaining in E15 DRG. TrkC-positive neurons normally eliminated in NT-3 KOs are rescued in double KOs.
(B) TrkA/TrkC immunostaining at P0 in DRG.
(C) PV immunostaining in P0 DRG. Rescued TrkC-positive cells fail to express PV.
(D) Ratio of TrkA-immunopositive cells to TrkC-immunopositive cells in E15 and P0 DRGs. Data are presented as percentage of cells with standard deviation. Double KOs always had similar ratios to Bax KOs, and NT-3 KOs had the least amount of TrkC-positive cells, if any.
(E) TrkA/TrkC immunostaining in E15 spinal cord. Arrow points to group Ia fibers. Dorsal is up.
Scale bar: 50 μm (A–C), 1 mm (E).