We thank Gail Martin for the Cre construct (plasmid pML78) and Oliver Bogler for the IRES-hPLAP construct (plasmid 1726); Bjorn Olsen and Benoit de Crombrugghe for antibodies; the following individuals for in situ probe templates: Sophie Candille, Arend Sidow (Bmp2 and Bmp4), Vicki Rosen (Agg), and Bjorn Olsen (Col1a1); Véronique Lefebvre for Sox5 and Sox6 probe templates and useful discussions; Michelle Johnson for help with phenotypic assays on mice; Dr. Corrine Davis for help in evaluating synovial sections; Rebecca Rountree for Adobe Photoshop and Illustrator tips; and members of the Kingsley lab for helpful comments on the manuscript. This work was supported by an NIH predoctoral training grant (RR), a postdoctoral fellowship from the Arthritis Foundation (MS), and grants from the National Institutes of Health (DK). Dr. Kingsley is an associate investigator of the Howard Hughes Medical Institute. Conflicts of interest. The authors have declared that no conflicts of interest exist.
Author contributions. RBR, MS, and DMK conceived and designed the experiments. RBR and MEM performed the experiments. RBR, HC, and DMK analyzed the data. MS, HC, VH, and YM contributed reagents/materials/analysis tools. RBR and DMK wrote the paper.
Academic Editor: Lee Niswander, University of Colorado Health Sciences Center
¤ Current address: ARTEMIS Pharmaceuticals, an Exelixis Company, Köln, Germany
Citation: Rountree RB, Schoor M, Chen H, Marks ME, Harley V, et al. (2004) BMP receptor signaling is required for postnatal maintenance of articular cartilage. PLoS Biol 2(11): e355.
Abbreviations
BAC bacterial artificial chromosome
Bmpr1a bone morphogenetic protein receptor 1a
E[number] embyonic day [number]
ECM extracellular matrix
GAC transgenic line carrying Gdf5-alkaline phosphatase-Cre construct
Gdf5 growth differentiation factor 5
hPLAP human placental alkaline phosphatase
IRES internal ribosome entry site
PFA paraformaldehyde
R26R lacZ ROSA26 Cre reporter strain
TGF-β transforming growth factor β
TUNEL terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling