However, in Arctic charr, CART hypothalamic expression is similar throughout the seasonal feeding cycles (Striberny et al., 2015) and fasting does not affect CART expression in either dourado (Volkoff et al., 2016), winter flounder (MacDonald and Volkoff, 2009a) or Atlantic halibut larvae (Gomes et al., 2015), and in lake trout, fish exposed to the pesticide tebufenozide and control fish have similar food intakes, despite higher CART mRNA brain expression levels in exposed fish (Volkoff et al., 2007). In winter skate, 2 weeks of fasting have no effects on brain CART expression (MacDonald and Volkoff, 2009b), suggesting that CART might not have a major feeding-regulating role in elasmobranchs.