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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/5126056","sourcedb":"PMC","sourceid":"5126056","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5126056","text":"Research on the endocrine regulation of feeding in fish has progressed in recent years. New fish appetite-regulating hormones and species other than traditional models (such as goldfish, salmon and zebrafish) are gradually being examined. In addition, traditional techniques such as brain lesions and injections and biochemical purification of peptides, although still useful and being used, have been complemented by new approaches such as gene expression studies, quantitative PCR, genomics (microarrays, RNA-seq), proteomics and metabolomics, transgenesis, gene knockout and silencing, and in vitro (cell and tissue culture, perifusion) studies.","tracks":[]}