Box Plot-Whiskers Graphs Box plots with notches were created using the web tool at http://boxplot.tyerslab.com/. Center line shows the median; box limit indicates the 25th and 75th percentiles as determined by R software; whiskers extend to minimum and maximum of the values; crosses indicate sample means (26, 27). The notches are defined as ±1.58× interquartile range per square root (n) and represent the 95% confidence interval for each median.