Abbreviations: Real-time reverse transcription-PCR, rRT-PCR; spring viraemia of carp virus, SVCV. Individual animals are specified by their identity number (Animal ID); SVCV confirmation of isolate (SVCV Isolate: Yes or No); and SVCV rRT-PCR test status of tissues from animals that were previously pooled for virus isolation (Positive or Not applicable). Viral isolates were each identified by PCR testing and sequencing of a portion of the G-gene. An asterisk indicates the animal from which the entire genome of SVCV isolate 202238 was sequenced.