Haplotype Analyses
To determine whether recurring MME mutations (c.654+1G>A) occurred because of independent mutational events or common ancestry, we performed a haplotype analysis using five microsatellite markers (D3S1595, D3S1280, D3S3509, D3S1275, and D3S3692) and seven single‐nucleotide polymorphisms (SNPs; rs1803155, rs12493885, rs12497267, rs9438, rs358733, rs11918974, and rs3816527) flanking the MME gene by an automated fluorescent method on an ABI 3130xL or 3500xL genetic analyzer (Applied Biosystems).