Figure 1  Reverse phase protein arrays (RPPA) experimentation involves (A) printing of samples in a neatly organized array format onto, for example, nitrocellulose-coated glass slides; (B) Incubation with a highly, target-specific primary antibody to detect proteins-of-interest, or a certain phosphorylation sites; (C) Signal detection of the primary antibody is commonly performed by fluorescence, chemiluminescence or colorimetric methods; (D) Target intensities are quantified after scanning and analyzing signal intensities of individual spots; (E) Data processing and quality control can be performed with the R-package RPPanalyzer (Table 1), for example.