Figure 2  Cell culture alters the transcriptional profile of adipose-derived stem cells. Differentially-expressed genes among primary, passage 0, and passage 1 cells identified using non-parametric two sample Kolmogorov–Smirnov testing. Twelve genes exhibit significantly different (p < 0.01 following Bonferroni correction for multiple comparisons) distributions of single cell expression between fresh→P0 or P0→P1 populations, illustrated here using median-centered Gaussian curve fits. The left bar for each panel represents the fraction of qPCR reactions that failed to amplify in each group. The curves above the dashed line represent transcriptional comparisons between primarily isolated cells versus cells at passage 0, whereas the curves below the dashed line represent comparisons between cells at passage 1 versus passage 0.