Phosphorylation and dephosphorylation are ubiquitous post-translational modifications of proteins. These processes, directed by protein kinases and phosphatases, cause conformational changes in the target protein, and lead to the regulation of protein function [1]. Since the functional activities of many proteins are interrelated, phosphorylation events may lead to complex signalling pathways that are involved in and coordinate those activities. To gain accurate knowledge about these complex signalling pathways and regulation mechanisms, it is essential to measure phosphorylation activities. Kinome microarray technology allows measurement of the phosphorylation activity of hundreds of proteins in a single experiment [2,3,4].