Figure 3  Comparison of protein expressions in the same tissue cores before (1) and after re-punching (2): (A) Pan-keratin in epithelial carcinoma cells (1mm diameter core); (B) Ki67 in epithelial carcinoma cells (1 mm diameter core); (C) Actin positivity of smooth muscle (0.6 mm diameter core); (D) Colon with blood vessels positive for CD34 (0.6 mm diameter core); and (E) Thyroid carcinoma with CD45 positive lymphocytes (1 mm diameter core).