Figure 5  RPPA-spotting pattern detected by chemiluminescent detection. Subgrids comprise 6 samples, each in a five-spot serial dilution plus protein extraction buffer as negative control (36 spots). Grids comprise 16 subgrids and 576 spots (Grid 1–3)/12 subgrids and 432 spots (Grid 4). In total, 180 samples can be spotted per slide (2160 spots). Numbers (1–3) indicate three samples spotted in duplicates. * Smaller grid (3/4 size of Grids 1–3).