Figure 3  Antibody validation by Western Blot using different cell lines. The left panel shows an antibody that is suitable for RPPA analysis as it detects one single protein band at the expected molecular weight. The antibody used for the right panel should not be used for RPPA studies as there are numerous bands besides the proposed “specific” band. 1–4: lysates from breast cancer FFPE-tissue; 5–8: lysates from different cell lines; arrows indicate the expected molecular weight.