3.1. Information Obtained for Interpreting Virtual Hybridization Results
Information produced by the Virtual Hybridization software is stored in an output file in text format; this includes: file name, total number of genomes hybridized, number of experimental probes, name of the file where the probes set is stored, name of the genome hybridized, number of times that the same probe hybridized across the genome, probe number, probe sequence, position in the genome where the probe hybridized, complementary sequence, and Δ°G between the probe and the target sequence. Comparison of the virtual hybridization patterns obtained with the direct method yielded an average of 1528 potential hybridization sites, and 4603 for the extended method (Table 2).
microarrays-04-00084-t002_Table 2 Table 2  Number of signals obtained with direct or extended methods for Bacillus anthracis.   * Upon virtual hybridization with 15,264 probes, using a Δ G° value between −19.53 and −11.67 (kcal/mol). ** Upon virtual hybridization with 15,264 probes, using a Δ G° value between −19.53 and −13.01 (kcal/mol).

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