2.2. CMA Construction
Galileo TMA CK4500 platform (Isenet, Milan, Italy) was used for CMA construction. A hollow needle of 1mm diameter was used to pick cell cores from the donor paraffin block, which were then assembled in the recipient block. Up to 80 consecutive sections of 5 µm thickness can be cut from the CMA block and mounted on microscope slides. The slides were now ready to be assayed with different stemness and differentiation markers.