Figure 3  Immunocharacterization of the AF22 iPS-derived cell line by CMA technology versus coverslip. Undifferentiated (A) and differentiated AF22 cells (B) grown on coverslip were analyzed for Nestin, Sox2 and β3-tubulin expression by immunofluorescence. Undifferentiated cells were uniformly immunopositive for the neural precursor cell markers Nestin, Sox2 and SEL1L, a protein involved in neural lineage commitment but negative for the neuronal differentiation markers β3-tubulin. A clear decrease of Nestin, Sox2 and SEL1L is shown in the differentiated (B) cells with the concomitant increase of β3-tubulin expression. AF22 cells grown in stemness and differentiated conditions were simultaneously grown in flasks and collected either by trypsinization (C) or with a rubber policemen (D). Scale bars: 100 µm.