Immunoblotting
Cell extracts were prepared by lysing cells in urea buffer (9 M urea, 50 mM Tris HCL, pH 7.3, 150 mM β-mercaptoethanol) followed by sonication using a soniprep 150 (MSE) probe sonicator. Proteins were resolved by SDS-PAGE and transferred to PVDF. Immunoblots were carried out using the indicated antibodies (See Supplementary Table 1)