2.4. Lymphoblast Cell Line (LCL) qPCR Biomarker (Experiment 4)
In addition to testing brain samples by qPCR (Experiment 2), 87 EBV-transformed lymphoblast cell lines (LCLs) from Costa Rica were tested for expression levels of the three MHC Class II genes via qPCR. Previously transformed cell lines were grown to confluency and harvested for RNA using Trizol. cDNA was synthesized as described above (Table 3, demographics).