We define the marker with the greatest contribution to the mini network disruption as the potential vital biomarker during the disease progression, and its contribution can be measured by the probability of mini network disruption calculated when only a single marker or a group of markers with interaction is disturbed. For the recognition of potential vital biomarkers, the first step of Monte Carlo simulation needs a minor modification. When evaluating the contribution of the ith marker, it needs to be disturbed and the other markers remain invariant. The joint contribution of multi-marker can be estimated in the same way: disturb the group of the markers to be evaluated and keep the other markers constant.