Additional file
Additional file 1: Figure S1.  Analysis of tissue water content. Tissue water content in the investigated tissue structure of the brain and in the trigeminal ganglia is shown. Tissue water content following application of CFA or IS to the dura was compared to brain water content following application of vehicle to the dura. *p ≤0.05. (TIFF 515 kb) Abbreviations
BBB Blood - Brain Barrier
CFA Complete Freund’s Adjuvant
CGRP Calcitonin Gene-Related Peptide
CNS Central Nervous System
EBA Evans Blue Albumin
IL InterLeukin
IS Inflammatory soup
MAP Mean Arterial blood Pressure
MMA Middle Meningeal artery
PAG PeriAqueductal Gray
PS Permeability Surface area product
TG Trigeminal Ganglion
TNC Trigeminal Nucleus Caudalis
Competing interests
The authors CL, KAH and GG declare no conflict of interest. LE has consulted for TEVA Pharma and Eli Lilly Pharma.
Authors’ contributions
CL performed the Tissue Water Content and Permeability surface experiments combined with analysis and helped to draft the manuscript. KAH performed the Evans blue and Photo microscopy and drafted the manuscript. GG contributed in the experiments and analysis. LE conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

Funding
This work was supported by the Lundbeck foundation; Lundbeck Grant of excellence, the heart and lung foundation, the Swedish Research Council, Region Skåne (ALF) and the Medical Faculty of Lund University, Sweden. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.