Figure 17 Tissue-specific upregulation of RNF213 in EC-Tg and SMC-Tg mice. Protein extraction from purified ECs from lungs of EC-Mut Tg and WT mice (left panel) and protein extraction from aorta of SMC-Mut Tg and WT mice (right panel) were immunoblotted. β-tubulin served as the loading control. Similar results were obtained from 3 independent experiments. ECs indicates endothelial cells; SMC, smooth muscle cells; Tg, transgenic; WT, wild type. F