Figure 12  Tube formation assays for HUVECs after 20 hours of culture on Matrigel with IFN-β after control or RNF213 siRNA transfection. Treatment with 0 ng/mL of IFN-β after control siRNA transfection served as the positive control (100%). Total tube length and number of branches were quantified by imaging analysis. A column with a bar represents means±SD (n=3). *P<0.05, by Student t test compared with 0 ng/mL of IFN-β within the same siRNA treatment. HUVECs indicates human umbilical vein endothelial cells; IFN, interferon; iPSECs, induced pluripotent stem cell-derived vascular endothelial cells.