Figure 8  Antiangiogenic activity of IFN-β in HUVECs. A, Tube formation assays in HUVECs after 20 hours of culture with IFN-β on Matrigel. A concentration of 0 ng/mL of IFN-β was used as a positive control (100%). Scale bars indicate 100 μm. Representative images are shown in left panel. The tubal areas were quantified by imaging analysis (right panel). Data with bars represent mean±SD (n=3). *P<0.05 according to Student t test compared with 0 ng/mL of IFN-β. B, Migration assays for HUVECs after treatment with IFN-β (1 ng/mL). A concentration of 0 ng/mL of IFN-β was used as a control. Scale bars indicate 100 μm. Representative images are shown in left panel. Re-endothelialized areas were quantified by imaging analysis (right panel). Data with bars represent mean±SD (n=3). *P<0.05 according to Student t test compared with 0 ng/mL of IFN-β. HUVECs indicates human umbilical vein endothelial cells; IFN, interferon.
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