Figure 5  Effects of IFNs on RNF213 protein expression in HUVECs. HUVECs were treated with various concentrations of IFNs for 24 hours, and RNF213 protein expression was examined by western blotting analysis using β-tubulin as a loading control. Representative western blotting findings are shown in upper panel. A column represents a mean of 3 independent experiments (lower panel). Bars indicate SD. *P<0.05, by Student t test compared with 0 ng/mL IFNs. HUVECs indicates human umbilical vein endothelial cells; IFNs, interferons.