Figure 4  Effect of the PEX1 and PEX6 Mutations on Peroxisome Biogenesis
Fibroblasts deficient in PEX1 or PEX6 were transfected with a peroxisomal fluorescent marker and expression plasmids containing the constructed PEX variants. The ratio of complemented cells was quantified per construct and normalized to the maximal complementation capability as measured in co-transfections of peroxisomal markers with the functional PEX versions (positive controls set as 100%). n = 5–7 per construct (mean ± SEM); ∗p < 0.05 (statistical analysis by one-sample Wilcoxon signed-rank test for which the null hypothesis, H0, was that the median sample value equals the maximal complementation capability [100%], which is the ratio of peroxisome-positive cells to wild-type PEX-complemented cells).