Peroxisomal Parameters of Individuals with HS The finding of biallelic variants in PEX1 and PEX6 in the six HS-affected families suggested a peroxisomal defect in the affected individuals. We therefore analyzed plasma and erythrocytes from affected individuals with PEX1 and PEX6 variants (F1-II:3, F5-II:2, and F5-II:3) for peroxisomal parameters. These were all within the normal range and did not indicate peroxisomal dysfunction. Moreover, we also did not identify any significant peroxisomal biochemical aberrations in cultured skin fibroblasts from affected individuals F1-II:3 and F5-II:2 (Tables 3 and 4). However, previous studies have shown that individuals with very mild PBDs do not necessarily demonstrate significant biochemical abnormalities in plasma and/or fibroblasts.26,27 Thus, on the basis of these biochemical findings, we could not exclude a peroxisomal defect. Other evidence of clinical effect due to peroxisome dysfunction was not investigated because there was no clinical indication.