Figure 1  Dual-channel screening strategy and controls. (A) Living integrin αvβ3-positive M21 cells (target cells; stained with ESNF10 and pseudo-colored in red) and integrin αvβ3‑negative M21-L cells (control cells; stained with IR786 and pseudo-colored in green) prior to dissociation from their respective plates. Scale bars = 100 μm. (B) The same cells mixed together and panned over PAAm positive control spots (top row) or cRGDyK ligand spots (bottom row). The yellow color indicates co-localized M21 and M21-L cells. Scale bars = 300 μm.